Sizing of single fluorescently stained DNA fragments by scanning microscopy

We describe an approach to determine DNA fragment sizes based on the fluorescence detection of single adsorbed fragments on specifically coated glass cover slips. The brightness of single fragments stained with the DNA bisintercalation dye TOTO-1 is determined by scanning the surface with a confocal...

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Bibliografski detalji

Izdano u:	Nucleic Acids Res
Glavni autori:	Laib, Stephan, Rankl, Michael, Ruckstuhl, Thomas, Seeger, Stefan
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 2003
Teme:	NAR Methods Online
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC275575/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14602931/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng138
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