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Sizing of single fluorescently stained DNA fragments by scanning microscopy
We describe an approach to determine DNA fragment sizes based on the fluorescence detection of single adsorbed fragments on specifically coated glass cover slips. The brightness of single fragments stained with the DNA bisintercalation dye TOTO-1 is determined by scanning the surface with a confocal...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC275575/ https://ncbi.nlm.nih.gov/pubmed/14602931 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng138 |
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