Sizing of single fluorescently stained DNA fragments by scanning microscopy

We describe an approach to determine DNA fragment sizes based on the fluorescence detection of single adsorbed fragments on specifically coated glass cover slips. The brightness of single fragments stained with the DNA bisintercalation dye TOTO-1 is determined by scanning the surface with a confocal...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Laib, Stephan, Rankl, Michael, Ruckstuhl, Thomas, Seeger, Stefan
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2003
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC275575/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14602931/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng138
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