Sizing of single fluorescently stained DNA fragments by scanning microscopy

We describe an approach to determine DNA fragment sizes based on the fluorescence detection of single adsorbed fragments on specifically coated glass cover slips. The brightness of single fragments stained with the DNA bisintercalation dye TOTO-1 is determined by scanning the surface with a confocal...

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Dettagli Bibliografici

Pubblicato in:	Nucleic Acids Res
Autori principali:	Laib, Stephan, Rankl, Michael, Ruckstuhl, Thomas, Seeger, Stefan
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2003
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC275575/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14602931/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng138
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