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Structural redesign of lipase B from Candida antarctica by circular permutation and incremental truncation
Circular permutation of Candida antarctica lipase B yields several enzyme variants with substantially increased catalytic activity. To better understand the structural and functional consequences of protein termini reorganization, we have applied protein engineering and x-ray crystallography to cp28...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2009
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2753740/ https://ncbi.nlm.nih.gov/pubmed/19683009 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.08.008 |
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