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Structural redesign of lipase B from Candida antarctica by circular permutation and incremental truncation

Circular permutation of Candida antarctica lipase B yields several enzyme variants with substantially increased catalytic activity. To better understand the structural and functional consequences of protein termini reorganization, we have applied protein engineering and x-ray crystallography to cp28...

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Autori principali: Qian, Zhen, Horton, John R., Cheng, Xiaodong, Lutz, Stefan
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2753740/
https://ncbi.nlm.nih.gov/pubmed/19683009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.08.008
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