Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen

We describe herein a protocol for the production of antigen-specific human monoclonal antibodies (hmAbs). Antibody-secreting cells (ASCs) are isolated from whole blood collected 7 d after vaccination and sorted by flow cytometry into single cell plates. The antibody genes of the ASCs are then amplif...

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Detaylı Bibliyografya
Asıl Yazarlar: Smith, Kenneth, Garman, Lori, Wrammert, Jens, Zheng, Nai-Ying, Capra, J Donald, Ahmed, Rafi, Wilson, Patrick C
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2009
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2750034/
https://ncbi.nlm.nih.gov/pubmed/19247287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.3
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