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Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen
We describe herein a protocol for the production of antigen-specific human monoclonal antibodies (hmAbs). Antibody-secreting cells (ASCs) are isolated from whole blood collected 7 d after vaccination and sorted by flow cytometry into single cell plates. The antibody genes of the ASCs are then amplif...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2750034/ https://ncbi.nlm.nih.gov/pubmed/19247287 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.3 |
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