Direct Observation of Downhill Folding of λ-Repressor in a Microfluidic Mixer

The protein λ(6-85) has been implicated in barrierless folding by observations of kinetic relaxation after nanosecond T-jump. In this work we observed folding of this protein after dilution of a high denaturant in an ultrarapid microfluidic mixer at temperatures far below the thermal midpoint. The o...

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Hlavní autoři: DeCamp, Stephen J., Naganathan, Athi N., Waldauer, Steven A., Bakajin, Olgica, Lapidus, Lisa J.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2009
Témata:
Protein
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2749784/
https://ncbi.nlm.nih.gov/pubmed/19751683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.07.003
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