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Pressure-Dependent Structure Changes in Barnase on Ligand Binding Reveal Intermediate Rate Fluctuations

In this work we measured (1)H NMR chemical shifts for the ribonuclease barnase at pressures from 3 MPa to 200 MPa, both free and bound to d(CGAC). Shift changes with pressure were used as restraints to determine the change in structure with pressure. Free barnase is compressed by ∼0.7%. The largest...

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Main Authors: Wilton, David J., Kitahara, Ryo, Akasaka, Kazuyuki, Pandya, Maya J., Williamson, Mike P.
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2009
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2749754/
https://ncbi.nlm.nih.gov/pubmed/19720037
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.06.022
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