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Systematical Optimization of Reverse-phase Chromatography for Shotgun Proteomics
We report the optimization of a common LC/MS/MS platform to maximize the number of proteins identified from a complex biological sample. The platform uses digested yeast lysate on a 75 μm internal diameter × 12 cm reverse-phase column that is combined with an LTQ-Orbitrap mass spectrometer. We first...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2009
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2749476/ https://ncbi.nlm.nih.gov/pubmed/19566079 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr900251d |
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