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Optimization of a Genome-Wide Disordered Lentivector-Based Short Hairpin RNA Library

To obtain a whole genome library that suppresses the total diversity of human mRNAs, lentiviral vector constructs and a short hairpin RNA (shRNA) expression cassette were optimized. The optimization of the vector increased the virus titer in preparations by 15-20 times. A simple shRNA structure with...

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Bibliografske podrobnosti
Main Authors: Guryanova, O.A., Makhanov, M., Chenchik, A.A., Chumakov, P.M., Frolova, E.I.
Format: Artigo
Jezik:Inglês
Izdano: 2006
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2748426/
https://ncbi.nlm.nih.gov/pubmed/19777129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1134/S002689330603006X
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