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Optimization of a Genome-Wide Disordered Lentivector-Based Short Hairpin RNA Library
To obtain a whole genome library that suppresses the total diversity of human mRNAs, lentiviral vector constructs and a short hairpin RNA (shRNA) expression cassette were optimized. The optimization of the vector increased the virus titer in preparations by 15-20 times. A simple shRNA structure with...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2006
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2748426/ https://ncbi.nlm.nih.gov/pubmed/19777129 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1134/S002689330603006X |
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