Identification and Quantification of Glycoproteins Using Ion-Pairing Normal-phase Liquid Chromatography and Mass Spectrometry

Glycoprotein structure determination and quantification by MS requires efficient isolation of glycopeptides from a proteolytic digest of complex protein mixtures. Here we describe that the use of acids as ion-pairing reagents in normal-phase chromatography (IP-NPLC) considerably increases the hydrop...

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Autori principali: Ding, Wen, Nothaft, Harald, Szymanski, Christine M., Kelly, John
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Biochemistry and Molecular Biology 2009
Soggetti:
Research
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2742440/
https://ncbi.nlm.nih.gov/pubmed/19525481
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M900088-MCP200
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