An Azido-Biotin Reagent for Use in the Isolation of Protein Adducts of Lipid-derived Electrophiles by Streptavidin Catch and Photorelease

HNE (4-hydroxynonenal), a byproduct of lipid peroxidation, reacts with nucleophilic centers on proteins. A terminal alkynyl analog of HNE (alkynyl HNE, aHNE) serves as a surrogate for HNE itself, both compounds reacting with protein amine and thiol functional groups by similar chemistry. Proteins mo...

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Detalhes bibliográficos
Main Authors: Kim, Hye-Young H., Tallman, Keri A., Liebler, Daniel C., Porter, Ned A.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2009
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2742437/
https://ncbi.nlm.nih.gov/pubmed/19483245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M900121-MCP200
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