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Two-Dimensional Southwestern Blotting and Characterization of Transcription Factors On-blot
Two-dimensional Southwestern blotting (2D-SW) described here combines several steps. Proteins are separated by two-dimensional gel electrophoresis and transferred to nitrocellulose (NC) or polyvinylidene fluoride (PVDF) membrane. The blotted proteins are then partially renatured and probed with a sp...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2738868/ https://ncbi.nlm.nih.gov/pubmed/19388704 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr900214p |
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