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Asymmetric PCR provides alternatives for preparation of (GT)(5) tailed duplex DNA promoter for promoter trapping
Synthesis of (GT)(5) tailed duplex DNA promoter is an important first step for purifying transcription complexes by promoter trapping purification. In our earlier publication we have shown that the purification of the c-jun promoter using lambda exonuclease digestion of PCR produced DNA with single...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2012
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3398248/ https://ncbi.nlm.nih.gov/pubmed/22633908 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.05.013 |
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