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Asymmetric PCR provides alternatives for preparation of (GT)(5) tailed duplex DNA promoter for promoter trapping

Synthesis of (GT)(5) tailed duplex DNA promoter is an important first step for purifying transcription complexes by promoter trapping purification. In our earlier publication we have shown that the purification of the c-jun promoter using lambda exonuclease digestion of PCR produced DNA with single...

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Bibliografske podrobnosti
Main Authors: Zhou, YanWen, Jia, Yinshan, Jarrett, Harry W.
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3398248/
https://ncbi.nlm.nih.gov/pubmed/22633908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.05.013
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