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Rescue of Bacteriophage T7 DNA Polymerase of Low Processivity by Suppressor Mutations Affecting Gene 3 Endonuclease

The DNA polymerase encoded by gene 5 (gp5) of bacteriophage T7 has low processivity, dissociating after the incorporation of a few nucleotides. Upon binding to its processivity factor, Escherichia coli thioredoxin (Trx), the processivity is increased to approximately 800 nucleotides per binding even...

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Detalhes bibliográficos
Main Authors: Lee, Seung-Joo, Chowdhury, Kajal, Tabor, Stanley, Richardson, Charles C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2738204/
https://ncbi.nlm.nih.gov/pubmed/19535436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00855-09
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