Detection and Genotyping of Human Rotavirus VP4 and VP7 Genes by Reverse Transcriptase PCR and Reverse Hybridization

Rotavirus infections can be diagnosed in stool samples by serological and molecular methods. We developed a novel reverse transcriptase PCR (RT-PCR) method for the amplification of rotavirus RNA and a reverse hybridization assay on a strip to detect amplimers and identify the specific G and P genoty...

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Bibliographic Details
Main Authors: van Doorn, Leen-Jan, Kleter, Bernhard, Hoefnagel, Evert, Stainier, Isabelle, Poliszczak, Annick, Colau, Brigitte, Quint, Wim
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
Subjects:
Virology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2738096/
https://ncbi.nlm.nih.gov/pubmed/19553575
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00378-09
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