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Evaluation of a Novel Highly Sensitive, Broad-Spectrum PCR-Reverse Hybridization Assay for Detection and Identification of Beta-Papillomavirus DNA

Human papillomavirus can be detected by amplification of viral DNA. A novel one-step PCR (PM-PCR) was evaluated for amplification of a 117-bp fragment from the E1 region. It permitted ultrasensitive detection of all 25 known human papillomavirus genotypes from the beta-papillomavirus genus. The intr...

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Detalhes bibliográficos
Main Authors: de Koning, Maurits, Quint, Wim, Struijk, Linda, Kleter, Bernhard, Wanningen, Patrick, van Doorn, Leen-Jan, Weissenborn, Sönke Jan, Feltkamp, Mariet, ter Schegget, Jan
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1479195/
https://ncbi.nlm.nih.gov/pubmed/16672409
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.44.5.1792-1800.2006
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