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New yeast recombineering tools for bacteria

Recombineering with Saccharomyces cerevisiae is a powerful methodology that can be used to clone multiple unmarked pieces of DNA to generate complex constructs with high efficiency. Here we introduce two new tools that utilize the native recombination enzymes of S. cerevisiae to facilitate the const...

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Bibliografiska uppgifter
Huvudupphovsmän: Shanks, Robert M. Q., Kadouri, Daniel E., MacEachran, Daniel P., O’Toole, George A.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2009
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737453/
https://ncbi.nlm.nih.gov/pubmed/19477196
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2009.05.002
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