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New yeast recombineering tools for bacteria
Recombineering with Saccharomyces cerevisiae is a powerful methodology that can be used to clone multiple unmarked pieces of DNA to generate complex constructs with high efficiency. Here we introduce two new tools that utilize the native recombination enzymes of S. cerevisiae to facilitate the const...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2737453/ https://ncbi.nlm.nih.gov/pubmed/19477196 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2009.05.002 |
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