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Quantitative Production of Compound I from a Cytochrome P450 Enzyme at Low Temperatures. Kinetics, Activation Parameters, and Kinetic Isotope Effects for Oxidation of Benzyl Alcohol

Cytochrome P450 enzymes are commonly thought to oxidize substrates via an iron(IV)-oxo porphyrin radical cation transient termed Compound I, but kinetic studies of P450 Compounds I are essentially non-existent. We report production of Compound I from cytochrome P450 119 (CYP119) in high conversion f...

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Detalhes bibliográficos
Main Authors: Wang, Qin, Sheng, Xin, Horner, John H., Newcomb, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737099/
https://ncbi.nlm.nih.gov/pubmed/19572732
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja9031105
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