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Thermodissociation of staphylococcal immune complexes and detection of staphylococcal antigen in serum from patients with Staphylococcus aureus bacteremia.
We describe a simple and rapid method to dissociate antigen-antibody complexes to allow antigen detection by radioimmunoassay. Immunoglobulins and immune complexes were precipitated from serum in 50%-saturated ammonium sulfate. The precipitate was dissolved in distilled water and heated at 90 degree...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1980
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC273490/ https://ncbi.nlm.nih.gov/pubmed/7430337 |
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