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Thermodissociation of staphylococcal immune complexes and detection of staphylococcal antigen in serum from patients with Staphylococcus aureus bacteremia.

We describe a simple and rapid method to dissociate antigen-antibody complexes to allow antigen detection by radioimmunoassay. Immunoglobulins and immune complexes were precipitated from serum in 50%-saturated ammonium sulfate. The precipitate was dissolved in distilled water and heated at 90 degree...

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Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Tabbarah, Z A, Wheat, L J, Kohler, R B, White, A
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1980
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC273490/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7430337/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.11.6.703-709.1980
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