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A Simple Procedure for Effective Quenching of Trypsin Activity and Prevention of (18)O-Labeling Back-Exchange
Trypsin-catalyzed stable isotope (16)O/(18)O-labeling of the C-terminal carboxyl groups of peptides is increasingly used in shotgun proteomics for relative peptide/protein quantitation. However, precise quantitative measurements are often complicated by residual trypsin that can catalyze the back-ex...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2728467/ https://ncbi.nlm.nih.gov/pubmed/19222237 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr800971w |
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