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The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry

The success in profiling the phosphoproteome by mass spectrometry-based proteomics has been intimately related to the availability of methods that selectively enrich for phosphopeptides. To this end, we describe a protocol that combines two sequential enrichment steps. First, strong cation exchange...

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Hlavní autoři: Villén, Judit, Gygi, Steven P
Médium: Artigo
Jazyk:Inglês
Vydáno: 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2728452/
https://ncbi.nlm.nih.gov/pubmed/18833199
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.150
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