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The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
The success in profiling the phosphoproteome by mass spectrometry-based proteomics has been intimately related to the availability of methods that selectively enrich for phosphopeptides. To this end, we describe a protocol that combines two sequential enrichment steps. First, strong cation exchange...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2728452/ https://ncbi.nlm.nih.gov/pubmed/18833199 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.150 |
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