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Conversion of MDCK cell line to suspension culture by transfecting with human siat7e gene and its application for influenza virus production

MDCK cells are currently being considered as an alternative to embryonated eggs for influenza virus propagation and hemagglutinin (HA) production intended for vaccine manufacturing. MDCK cells were found suitable for the virus production but their inability to grow in suspension burdens the process...

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Autors principals: Chu, Chia, Lugovtsev, Vladimir, Golding, Hana, Betenbaugh, Michael, Shiloach, Joseph
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2728112/
https://ncbi.nlm.nih.gov/pubmed/19706449
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0905912106
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