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C-5(6) Sterol Desaturase from Tetrahymena thermophila: Gene Identification and Knockout, Sequence Analysis, and Comparison to Other C-5(6) Sterol Desaturases

The gene coding for a C-5(6) sterol desaturase in Tetrahymena thermophila, DES5A, has been identified by the knockout of the TTHERM_01194720 sequence. Macronucleus transformation was achieved by biolistic bombardment and gene replacement through phenotypic assortment, using paromomycin as the select...

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Detalhes bibliográficos
Main Authors: Nusblat, Alejandro D., Najle, Sebastián R., Tomazic, Mariela L., Uttaro, Antonio D., Nudel, Clara B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2725563/
https://ncbi.nlm.nih.gov/pubmed/19525418
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00057-09
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