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Kinetic and thermodynamic characterization of single-mismatch discrimination using single-molecule imaging
A single-molecule detection setup based on total internal reflection fluorescence (TIRF) microscopy has been used to investigate association and dissociation kinetics of unlabeled 30mer DNA strands. Single-molecule sensitivity was accomplished by letting unlabeled DNA target strands mediate the bind...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2724293/ https://ncbi.nlm.nih.gov/pubmed/19509313 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp487 |
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