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Elucidating in Vivo Structural Dynamics in Integral Membrane Protein by Hydroxyl Radical Footprinting

We describe here a novel footprinting technique to probe the in vivo structural dynamics of membrane protein. This method utilized in situ generation of hydroxyl radicals to oxidize and covalently modify biomolecules on living Escherichia coli cell surface. After enriching and purifying the membrane...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Zhu, Yi, Guo, Tiannan, Park, Jung Eun, Li, Xin, Meng, Wei, Datta, Arnab, Bern, Marshall, Lim, Sai Kiang, Sze, Siu Kwan
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: The American Society for Biochemistry and Molecular Biology 2009
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2722762/
https://ncbi.nlm.nih.gov/pubmed/19473960
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M900081-MCP200
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