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Impact of Emission Anisotropy on Fluorescence Spectroscopy and FRET Distance Measurements
The objective of this report is to provide a practical and improved method for estimating Förster resonance energy transfer distance measurement error due to unknown angles in the dipole orientation factor based on emission anisotropy measurements. We improve on the method of Dale et al. (1979), whi...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2718156/ https://ncbi.nlm.nih.gov/pubmed/19651051 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.05.025 |
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