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Large-scale expression, purification, and characterization of an engineered prostacyclin-synthesizing enzyme with therapeutic potential

Recently, we reported that a novel hybrid enzyme (TriCat Enzyme), engineered by linking human cyclo-ooxygenase-2 (COX-2) with prostacyclin (PGI(2)) synthase (PGIS) together through a transmembrane domain, was able to directly integrate the triple catalytic (TripCat) functions of COX-2 and PGIS and e...

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Detalhes bibliográficos
Main Authors: Ruan, Ke-He, So, Shui-Ping, Wu, Hanjing, Cervantes, Vanessa
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2717720/
https://ncbi.nlm.nih.gov/pubmed/18835243
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2008.09.010
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