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Rapid, semi-automated, and inexpensive radioimmunoassay of cAMP: Application in GPCR-mediated adenylate cyclase assays

Cyclic AMP (cAMP) is an important signal transduction second messenger that is commonly used as a functional mirror on the actions of G protein-coupled receptors that can activate or inhibit adenylate cyclases. A radioimmunoassay for cAMP with femtomole sensitivity was first reported by Steiner more...

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Detalhes bibliográficos
Main Authors: Brown, Justin T., Kant, Andrew, Mailman, Richard B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2717699/
https://ncbi.nlm.nih.gov/pubmed/19007813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2008.10.016
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