SHAMS: Combining chemical modification of RNA with mass spectrometry to examine polypurine tract-containing RNA/DNA hybrids

Selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) has gained popularity as a facile method of examining RNA structure both in vitro and in vivo, exploiting accessibility of the ribose 2′-OH to acylation by N-methylisatoic anhydride (NMIA) in unpaired or flexible configurations. Su...

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Autors principals: Turner, Kevin B., Yi-Brunozzi, Hye Young, Brinson, Robert G., Marino, John P., Fabris, Daniele, Le Grice, Stuart F.J.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2009
Matèries:
Method
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2714758/
https://ncbi.nlm.nih.gov/pubmed/19535461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1615409
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