SHAMS: Combining chemical modification of RNA with mass spectrometry to examine polypurine tract-containing RNA/DNA hybrids

Selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) has gained popularity as a facile method of examining RNA structure both in vitro and in vivo, exploiting accessibility of the ribose 2′-OH to acylation by N-methylisatoic anhydride (NMIA) in unpaired or flexible configurations. Su...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Turner, Kevin B., Yi-Brunozzi, Hye Young, Brinson, Robert G., Marino, John P., Fabris, Daniele, Le Grice, Stuart F.J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2009
Schlagworte:
Method
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2714758/
https://ncbi.nlm.nih.gov/pubmed/19535461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1615409
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge