Purification of Capping Protein Using the Capping Protein Binding Site of CARMIL as an Affinity Matrix

Capping Protein (CP) is a ubiquitously expressed, heterodimeric actin binding protein that is essential for normal actin dynamics in cells. The existing methods for purifying native CP from tissues and recombinant CP from bacteria are time-consuming processes that involve numerous conventional chrom...

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Hlavní autoři: Remmert, Kirsten, Uruno, Takehito, Hammer, John A.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2009
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2713380/
https://ncbi.nlm.nih.gov/pubmed/19427903
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.05.002
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