Lipid Mixing and Content Release in Single-Vesicle, SNARE-Driven Fusion Assay with 1–5 ms Resolution

A single-vesicle, fluorescence-based, SNARE-driven fusion assay enables simultaneous measurement of lipid mixing and content release with 5 ms/frame, or even 1 ms/frame, time resolution. The v-SNARE vesicles, labeled with lipid and content markers of different color, dock and fuse with a planar t-SN...

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Detalles Bibliográficos
Main Authors: Wang, Tingting, Smith, Elizabeth A., Chapman, Edwin R., Weisshaar, James C.
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2009
Assuntos:
Membrane
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2712201/
https://ncbi.nlm.nih.gov/pubmed/19450483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.02.050
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