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Lipid Mixing and Content Release in Single-Vesicle, SNARE-Driven Fusion Assay with 1–5 ms Resolution
A single-vesicle, fluorescence-based, SNARE-driven fusion assay enables simultaneous measurement of lipid mixing and content release with 5 ms/frame, or even 1 ms/frame, time resolution. The v-SNARE vesicles, labeled with lipid and content markers of different color, dock and fuse with a planar t-SN...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The Biophysical Society
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2712201/ https://ncbi.nlm.nih.gov/pubmed/19450483 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.02.050 |
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