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Single Vesicle Assaying of SNARE-Synaptotagmin-Driven Fusion Reveals Fast and Slow Modes of Both Docking and Fusion and Intrasample Heterogeneity

Lipid mixing between vesicles functionalized with SNAREs and the cytosolic C2AB domain of synaptotagmin-1 recapitulates the basic Ca(2+) dependence of neuronal exocytosis. However, in the conventional ensemble lipid mixing assays it is not possible to discriminate whether Ca(2+) accelerates the dock...

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Bibliografski detalji
Glavni autori: Christensen, Sune M., Mortensen, Michael W., Stamou, Dimitrios G.
Format: Artigo
Jezik:Inglês
Izdano: The Biophysical Society 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3037598/
https://ncbi.nlm.nih.gov/pubmed/21320440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.12.3730
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