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Immuno-spin trapping analyses of DNA radicals

Immuno-spin trapping is a highly sensitive method for detecting DNA radicals in biological systems. This technique involves three main steps: (i) in situ and real-time trapping of DNA radicals with the nitrone spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), thus forming DMPO–DNA nitrone adducts (...

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Main Authors: Ramirez, Dario C, Gomez-Mejiba, Sandra E, Mason, Ronald P
格式: Artigo
語言:Inglês
出版: 2007
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2711897/
https://ncbi.nlm.nih.gov/pubmed/17406615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.5
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