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Intrinsic Dynamics of Restriction Endonuclease EcoO109I Studied by Molecular Dynamics Simulations and X-Ray Scattering Data Analysis

EcoO109I is a type II restriction endonuclease that functions as a dimer in solution. Upon DNA binding to the enzyme, the two subunits rotate counterclockwise relative to each other, as the two catalytic domains undergo structural changes to capture the cognate DNA. Using a 150-ns molecular dynamics...

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Detalhes bibliográficos
Main Authors: Oroguchi, Tomotaka, Hashimoto, Hiroshi, Shimizu, Toshiyuki, Sato, Mamoru, Ikeguchi, Mitsunori
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2711268/
https://ncbi.nlm.nih.gov/pubmed/19348764
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.12.3914
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