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Preparation of macromolecular complexes for cryo-electron microscopy

This protocol describes the preparation of frozen-hydrated single-particle specimens of macromolecular complexes. First, it describes how to create a grid surface coated with holey carbon by first inducing holes in a Formvar film to act as a template for the holey carbon that is stable under cryo-el...

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Main Authors: Grassucci, Robert A, Taylor, Derek J, Frank, Joachim
格式: Artigo
語言:Inglês
出版: 2007
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2710239/
https://ncbi.nlm.nih.gov/pubmed/18079724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.452
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