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Preparation of macromolecular complexes for cryo-electron microscopy
This protocol describes the preparation of frozen-hydrated single-particle specimens of macromolecular complexes. First, it describes how to create a grid surface coated with holey carbon by first inducing holes in a Formvar film to act as a template for the holey carbon that is stable under cryo-el...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2007
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2710239/ https://ncbi.nlm.nih.gov/pubmed/18079724 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2007.452 |
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