Highly efficient incorporation of the fluorescent nucleotide analogs tC and tC(O) by Klenow fragment

Studies of the mechanisms by which DNA polymerases select the correct nucleotide frequently employ fluorescently labeled DNA to monitor conformational rearrangements of the polymerase–DNA complex in response to incoming nucleotides. For this purpose, fluorescent base analogs play an increasingly imp...

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Detalles Bibliográficos
Main Authors: Sandin, Peter, Stengel, Gudrun, Ljungdahl, Thomas, Börjesson, Karl, Macao, Bertil, Wilhelmsson, L. Marcus
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2009
Assuntos:
Molecular Biology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2709563/
https://ncbi.nlm.nih.gov/pubmed/19401439
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp266
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