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Characterization of the Microsomal Cytochrome P450 2B4 O(2)-Activation Intermediates by Cryoreduction/EPR.

The oxy-ferrous complex of cytochrome P450 2B4 (2B4) has been prepared at − 40°C with and without bound substrate (butylated hydroxytoluene, BHT), and radiolytically oneelectron cryoreduced at 77K. EPR shows that in both cases the observed product of cryoreduction is the hydroperoxo-ferriheme specie...

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Bibliografiska uppgifter
Huvudupphovsmän: Davydov, Roman, Razeghifard, Reza, Im, Sang-Choul, Waskell, Lucy, Hoffman, Brian M.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2008
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2709102/
https://ncbi.nlm.nih.gov/pubmed/18700729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi800926x
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