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Characterization of the Microsomal Cytochrome P450 2B4 O(2)-Activation Intermediates by Cryoreduction/EPR.
The oxy-ferrous complex of cytochrome P450 2B4 (2B4) has been prepared at − 40°C with and without bound substrate (butylated hydroxytoluene, BHT), and radiolytically oneelectron cryoreduced at 77K. EPR shows that in both cases the observed product of cryoreduction is the hydroperoxo-ferriheme specie...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2709102/ https://ncbi.nlm.nih.gov/pubmed/18700729 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi800926x |
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