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Random dissection to select for protein split sites and its application in protein fragment complementation
To identify protein split sites quickly, a selection procedure by using chloramphenicol acetyl transferase (CAT) as reporter was introduced to search for folded protein fragments from libraries generated by random digestion and reassembly of the target gene, which yielded an abundant amount of DNA f...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Wiley Subscription Services, Inc., A Wiley Company
2009
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2708047/ https://ncbi.nlm.nih.gov/pubmed/19165722 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.42 |
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