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Purification and Reconstitution of Sterol Transfer by Native Mouse ABCG5 and ABCG8
ABCG5 (G5) and ABCG8 (G8) are ATP-binding cassette half-transporters that limit intestinal uptake and promote biliary secretion of neutral sterols. Here, we describe the purification of endogenous G5G8 from mouse liver to near homogeneity. We incorporated the native proteins into membrane vesicles a...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2707165/ https://ncbi.nlm.nih.gov/pubmed/18402465 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi800292v |
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