Effects of fixation on polymerase chain reaction detection of Mycobacterium leprae.

The effects of standard fixatives (10% neutral buffered formalin, ethanol and mercury based) on the detection of Mycobacterium leprae DNA by the polymerase chain reaction (PCR) were studied. Mercury-based fixatives (Zenker's and Carnoy-Lebrun's fluids) strongly inhibited PCR amplification of M. lepr...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Fiallo, P, Williams, D L, Chan, G P, Gillis, T P
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1992
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270594/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1452690/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.12.3095-3098.1992
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