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Effects of fixation on polymerase chain reaction detection of Mycobacterium leprae.

The effects of standard fixatives (10% neutral buffered formalin, ethanol and mercury based) on the detection of Mycobacterium leprae DNA by the polymerase chain reaction (PCR) were studied. Mercury-based fixatives (Zenker's and Carnoy-Lebrun's fluids) strongly inhibited PCR amplification...

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Bibliografiska uppgifter
Huvudupphovsmän: Fiallo, P, Williams, D L, Chan, G P, Gillis, T P
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1992
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC270594/
https://ncbi.nlm.nih.gov/pubmed/1452690
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