Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease.

DNA probes that hybridize to a mycobacterial insertion sequence, IS900, present in multiple copies in the genome of Mycobacterium paratuberculosis were found to be highly specific for M. paratuberculosis. DNA sequences derived from IS900 were used to prepare DNA primers for detection and identificat...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Vary, P H, Andersen, P R, Green, E, Hermon-Taylor, J, McFadden, J J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1990
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267840/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2351737/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.5.933-937.1990
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